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Surface Plasmon Resonance Imaging to Study the Molecular Mechanism of Alzheimer’s Disease

Authors:
Eric Finot
Antonin Ollagnier
Elizabeth Drolle
Zoya Leonenko

Keywords: SPR ; microfuidics ; biosensor ; Alzheimer's disease ; amyloid ; melatonin ; lipid membrane

Abstract:
We have developed a state-of-the-art surface plasmon resonance imaging (SPRi) apparatus combined with microfluidics and used it to detect the binding of amyloid beta peptides to the lipid membrane, an interaction known to be involved in the molecular mechanism of amyloid toxicity in Alzheimer’s disease, and to study protective effect of melatonin. Kinetics of binding was measured, and quantitative analysis was developed in order to detect melatonin partitioning into and amyloid binding to the membrane. We provided for the first time clear experimental evidence that the presence of melatonin in supported lipid membrane prevents the amyloid peptide from binding to the lipid membrane, thus gaining insight into the molecular mechanism of melatonin protection against amyloid toxicity in Alzheimer’s disease.

Pages: 1 to 4

Copyright: Copyright (c) IARIA, 2016

Publication date: April 24, 2016

Published in: conference

ISSN: 2519-836X

ISBN: 978-1-61208-523-4

Location: Venice, Italy

Dates: from April 24, 2016 to April 28, 2016