Home // BIOTECHNO 2015, The Seventh International Conference on Bioinformatics, Biocomputational Systems and Biotechnologies // View article
Authors:
Oleg N. Reva
Keywords: genetic barcoding; plant growth promoting; Bacillus; metagenomics.
Abstract:
Bacillus subtilis, B. amyloliquefaciens and other related strains isolated from rhizosphere are widely used in biotechnology as Plant Growth Promoting Rhizobacteria (PGPR) showing variable activity in different soils and on different plants. Despite diverse bioactivity, they are almost indistinguishable by phenotype and by 16S rRNA that makes it problematic to trace them down in nature. Spores of these microorganisms are abundant in many habitats, but it remains unclear whether these organisms thrive in these habitats or simply contaminated the samples? In this work, several new computational approaches were proposed for design, evaluation and application of multilocus genetic barcodes for identification and monitoring of biotechnological strains. The barcodes containing 150 marker genes were designed for 35 strains of the B. subtilis group and tested on publically available metagenomic datasets. Clear habitat preferences were observed for different subspecies and even individual strains. Also, an approach of evaluation and improving of sensibility and sensitivity of barcodes was proposed.
Pages: 16 to 20
Copyright: Copyright (c) IARIA, 2015
Publication date: May 24, 2015
Published in: conference
ISSN: 2308-4383
ISBN: 978-1-61208-409-1
Location: Rome, Italy
Dates: from May 24, 2015 to May 29, 2015