Home // BIOTECHNO 2015, The Seventh International Conference on Bioinformatics, Biocomputational Systems and Biotechnologies // View article
Authors:
Liberata Mwita
Wai Y Chan
Oleg N Reva
Sylvester L Lyantagaye
Svitlana V. Lapa
Lilija V. Avdeevad
Keywords: Bacillus atrophaeus; plant growth promoting; gene expression; transcriptional regulation.
Abstract:
Plant Growth Promoting Rhizobacteria (PGPR) are widely used in agriculture as an ecologically safe replacement of chemical pesticides and fertilizers. In this work, gene expression regulation under stimulation by maize root exudate was studied in a PGPR strain Bacillus atrophaeus UCMB-5137. A strong up-regulation of synthesis of (p)ppGpp (guanosine pentaphosphate) alarmone was observed. This alarmone is associated with the stringent response in bacteria, but its involvement in regulation of plant colonization has never been reported. Comparison of the profiles of gene expression at the classical stringent response and in the current experiment showed only a partial overlap that allowed us to make a conclusion that the tested bacteria had not been starved but responded specifically to the root exudate stimulation and (p)ppGpp alarmone is involved in this complex response. Comparison of the gene expression regulation in B. atrophaeus UCMB-5137 with a report obtained on a similar experiment with another PGPR strain B. amyloliquefaciens FZB42 showed that these two closely related organisms had used different strategies of plant colonization.
Pages: 23 to 26
Copyright: Copyright (c) IARIA, 2015
Publication date: May 24, 2015
Published in: conference
ISSN: 2308-4383
ISBN: 978-1-61208-409-1
Location: Rome, Italy
Dates: from May 24, 2015 to May 29, 2015