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Reconstitution of Ryanodine Receptor/Ca2+ Release Channels in S-layer Supported Lipid Membranes

Authors:
Vanessa-Dominique Larisch
Angelika Schrems
Uwe B. Sleytr
Martin Hohenegger
Bernhard Schuster

Keywords: biomimetics; supported lipid membrane; S-layer protein; functional reconstitution; ryanodine receptor/Ca2+ release channel.

Abstract:
A great challenge nowadays is the systematic study on medicinal relevant functional (trans)membrane proteins reconstituted in tunable biomimetic model lipid membranes. Ryanodine receptor/Ca2+ release channels (RyRs), integral membrane proteins present in various mammalian tissues, are of great importance for muscle contraction. For the present study, the RyR isoform 1 (RyR1) was isolated from the skeletal muscle of rabbits. Here, we show the reconstitution of RyR1 into an artificial model lipid system, the so-called S-layer supported lipid membrane (SsLM). Bacterial crystalline surface layer (S-layer) proteins render the possibility to generate solid supported membranes of high longevity on various supports in order to enable investigations by a great variety of surface-sensitive techniques. According to preliminary results, RyR1 was successfully reconstituted into the SsLM as proven by quartz crystal microbalance with dissipation monitoring. Moreover, control experiments revealed that RyR1 did not stick to an S-layer lattice without an attached lipid membrane. Hence, evidence is provided that the SsLM as multifunctional interface might constitute a pharmaceutically interesting platform to investigate, e.g., the effect of a broad range of drugs on reconstituted RyR1 or other membrane proteins in general.

Pages: 14 to 17

Copyright: Copyright (c) IARIA, 2014

Publication date: November 16, 2014

Published in: conference

ISSN: 2308-3530

ISBN: 978-1-61208-380-3

Location: Lisbon, Portugal

Dates: from November 16, 2014 to November 20, 2014