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Neural Cell Chip to Assess Toxicity Based on Spectroelectrochemical Technique

Authors:
Jeong-Woo Choi
Tae-Hyung Kim

Keywords: Toxicity assessment; Spectroelectrochemical method; Surface-enhanced Raman spectroscopy; Cell chip

Abstract:
A cell chip is an useful tool for the toxicity assessment of various kinds of chemicals, drugs or nanomaterials. Nanoscale film was fabricated on a conducting electrode surface to establish a cell-friendly environment which is effective for increasing cell adhesion, spreading and proliferations. Biofilm was further developed to three dimensional peptide nanopillar arrays which were more efficient than two dimensional peptide film in regard to various kinds of cellular functions. The electrochemical signals obtained from cells were found to be proportional to the cell viability which can be used as indicator for the toxicity evaluation of various kinds of toxic chemicals and nanomaterials quickly and sensitively. Surface-enhanced Raman spectroscopy (SERS) was further developed as powerful supplementary tool to identify cell cycles at different stage, to distinguish different cell lines, to discriminate alive- or dead- cells and to investigate toxicity of anticancer drugs on target cells. The combination of electrical detection and SERS technique was found to be excellent to investigate the changes of intracellular composition of cells, as well as to study the internal redox properties of single neural cell. Proposed cell chip based on spectroelectrochemical technique that combined electrochemical and SERS methods can be applied as an in vitro analysis tool in various kinds of biotechnology fields.

Pages: 5 to 8

Copyright: Copyright (c) IARIA, 2012

Publication date: August 19, 2012

Published in: conference

ISSN: 2308-3514

ISBN: 978-1-61208-208-0

Location: Rome, Italy

Dates: from August 19, 2012 to August 24, 2012