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Fluorescence Assay for Spore Germination Detection

Authors:
Andreas Tortschanoff
Sabine Lengger
Gerald Auböck
Cristina Consani

Keywords: germination; time resolved fluorescence, component; spore detection; sterilization detection.

Abstract:
Tracking viability of spores for assessing decontamination and sterilization quality is of utmost importance in medical and other clean environments. However, classical tests for spore contamination rely on bacterial growth, which is slow. Rather than monitoring bacterial growth, one can detect the spore germination process. Here, we present our method for reliable testing for spore germination by fluorescence detection of the associated DPA release. Extrapolating our results, we estimate that single spore detection will be possible with our method.

Pages: 41 to 42

Copyright: Copyright (c) IARIA, 2022

Publication date: October 16, 2022

Published in: conference

ISSN: 2308-3514

ISBN: 978-1-68558-006-3

Location: Lisbon, Portugal

Dates: from October 16, 2022 to October 20, 2022