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Mechanisms Controlling the Sensitivity of Amperometric Biosensors in the Case of Substrate and Product Inhibition

Authors:
Dainius Simelevicius
Romas Baronas

Keywords: modelling; simulation; apparent Michaelis constant; biosensor; inhibition

Abstract:
Special case of amperometric biosensors is investigated in this paper. Processes of substrate as well as product inhibition take place during the operation of these biosensors. The operation of biosensors is modelled by employing non-stationary reaction-diffusion equations containing a non-linear term related to non-Michaelis-Menten kinetics. The equation system is solved numerically using finite difference technique. Apparent Michaelis constant is chosen as a good indicator of biosensor reliability. Its dependency on the substrate and product inhibition as well as the diffusion modulus and the Biot number was investigated.

Pages: 61 to 66

Copyright: Copyright (c) IARIA, 2011

Publication date: October 23, 2011

Published in: conference

ISSN: 2308-4537

ISBN: 978-1-61208-169-4

Location: Barcelona, Spain

Dates: from October 23, 2011 to October 29, 2011