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Simulation of Bacterial Self-Organization in Circular Container Along Contact Line as Detected by Bioluminescence Imaging

Authors:
Romas Baronas

Keywords: chemotaxis; reaction-diffusion; pattern formation; whole-cell biosensor.

Abstract:
Simulation of quasi-one dimensional spatiotemporal pattern formation along the three phase contact line in the fluid cultures of lux-gene engineered Escherichia coli is investigated in this paper. The numerical simulation is based on a one-dimensional-in-space mathematical model of a bacterial self-organization as detected by quasi-one-dimensional bioluminescence imaging. The pattern formation in a luminous E. coli colony was mathematically modeled by the nonlinear reaction-diffusion-chemotaxis equations. The numerical simulation was carried out using the finite difference technique. Regular oscillations as well as chaotic fluctuations similar to experimental ones were computationally simulated. The effect of the signal-dependent as well as density-dependent chemotactic sensitivity on the pattern formation was investigated. The simulations showed that a constant chemotactic sensitivity can be applied for modeling the formation of the bioluminescence patterns in a colony of luminous E. coli.

Pages: 148 to 153

Copyright: Copyright (c) IARIA, 2011

Publication date: October 23, 2011

Published in: conference

ISSN: 2308-4537

ISBN: 978-1-61208-169-4

Location: Barcelona, Spain

Dates: from October 23, 2011 to October 29, 2011