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Numerical Simulation and Analysis of Enzyme-Catalysed Substrate Conversion in a Microbioreactor

Authors:
Linas Petkevičius
Romas Baronas

Keywords: reaction-diffusion; Michaelis - Menten kinetics; microbioreactor; CSTR.

Abstract:
The paper presents a non-linear mathematical model for digital simulation of an enzyme loaded porous microreactor. The model is based on a system of reaction-diffusion equations, containing a non-linear term related to the Michaelis-Menten kinetics, and involves three regions: the enzyme microreactor where the enzyme reaction as well as mass transport by diffusion take place, a diffusion limiting region (the Nernst layer), where only the mass transport by diffusion takes place, and a convective region, where the analyte concentration is maintained constant. Assuming well stirred conditions, the influence of the thickness of the Nernst layer on the behaviour of the product emission, as well as the impact of the diffusion modulus and the Biot number has been numerically investigated. The simulation results showed that the Nernst layer must be taken into consideration when modelling micro-size bioreactors. The digital simulation was carried out using the finite difference technique.

Pages: 1 to 6

Copyright: Copyright (c) IARIA, 2017

Publication date: October 8, 2017

Published in: conference

ISSN: 2308-4537

ISBN: 978-1-61208-594-4

Location: Athens, Greece

Dates: from October 8, 2017 to October 12, 2017